Effects of progesterone on apoptosis and expression of p53 protein in rats during focal cerebral ischemia /reperfusion injury
- VernacularTitle:黄体酮防治大鼠缺血再灌注脑损伤中细胞凋亡及p53蛋白的变化
- Author:
Na LU
;
Chao LI
;
Dongliang LI
- Publication Type:Journal Article
- Keywords:
progesterone;
ischemia;
reperfusion injury;
apoptosis;
p53
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the neuroprotective effect and molecular mechanism of progesterone on reperfusion injury following ischemia. METHODS: The rats with transient middle cerebral artery occlusion(MACO) was described by Zea-longa for 2 h and reperfusion for 24 h. 48 male rats were divided randomly into 6 groups that were the sham, ischmia/reperfusion (I/R), dimethl sulfoxide (DMSO), and posttreatment, pretreatment, pre+posttreament with PROG groups. The immunohistochemistry staining and TUNEL reaction were used to facilitate the quantities of p53 protein and apoptosis in the brain tissuses. RESULTS: There were 26.25? 3.54 cells in p53 immunostaining in the I/R group, 22.88? 3.52 cells in DMSO group, 15.00? 2.07 cells in the pretreatment group, 16.75? 2.60 cells in the posttreatment group, and 10.38? 1.69 cells in the pre and posttreament group. The number of apoptosic cells was 1.88? 0.25 in the sham group, 41.38? 3.85 in the I/R group, 38.13? 5.69 in the DMSO group, 22.88? 2.70 in the pretreatment group, 25.63? 2.93 in the posttreatment group, and 20.88? 2.30 in the pre+posttreament group. The difference among drug management groups(pretreament, posttreament, and pre+posttreament)and control groups(I/R, DMSO)was significant (P
