Expression of chemokine CXCR4 and formyl peptide receptor FPR in rat C6 glioma cell line
- VernacularTitle:大鼠胶质瘤细胞系C6趋化因子受体CXCR4和甲酰肽受体FPR的表达
- Author:
Jianhong CHEN
- Publication Type:Journal Article
- Keywords:
glioma;
chemoattractant receptor;
cell differentiation;
angiogenic factor
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To detect the expression of chemoattractant receptors, CXCR4 and formyl peptide receptor (FPR), in rat glioma cell line C6 and to explore their relation to tumor differentiation and angiogenesis. Methods The expression of CXCR4 and FPR by C6 cells were detected with immunocytochemistry. The differentiatial degrees were also detected to analyze the relationship of chemoattractant receptors with angiogenesis and malignancy of glioma. Results C6 cells were of a long spindle shape, expressing high level of vimentin but relatively low level of glial fibrillary acidic protein (GFAP). C6 cells were positive for CXCR4 and FPR in the cytoplasm and strongly positive for vascular endothelial growth factor (VEGF). Conclusion C6 cell line is poorly differentiated and highly expresses chemoattractant receptors CXCR4 and FPR, both of which might be related to the angiogenic potentials.
