Study of the immunomodulatory effect of RDP1258 in vivo
- VernacularTitle:RDP1258免疫调节功能在大鼠体内实验研究
- Author:
Shanhong YI
- Publication Type:Journal Article
- Keywords:
RDP1258;
HLA-derived peptide;
mixed leukocyte reaction;
heme oxygenase-1
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the immunosuppressive function of a novel HLA-derived peptide, RDP1258, and to investigate the mechanisms in vivo. Methods A peptide derived from HLA, RDP1258, was chemically synthesized. The effects of the peptide on alloreactive cytotoxic activities of rat spleen cells in vivo were observed using ~(3)H-TDR method. As compared with the HO-1 inducer hemin and the HO-1 inhibitor zinc protoporphyrin (ZnPP), the HO enzyme activity was analyzed by enzyme chemistry method. Results The in vivo administration of the synthetic HLA-derived peptide can obviously inhibit the proliferation of rat spleen cells stimulated by isoantigen and mitogen, and resulted in upregulation of splenic heme oxygenase activity and rapid upregulation of HO-1 protein expression. Conclusion RDP1258 inhibits the proliferation of rat spleen cells induced by mitogen and isoantigen probably through enhancing the HO-1 activity.
