Effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 in human vascular smooth muscle cells induced by angiotensin Ⅱ
- VernacularTitle:恒磁场对血管紧张素Ⅱ作用下血管平滑肌细胞VCAM-1分泌与表达的抑制作用
- Author:
Hexiang CHENG
;
Lian ZHOU
;
Haichang WANG
- Publication Type:Journal Article
- Keywords:
electromagnetic fields;
angiotensin Ⅱ;
muscle, smooth, vascular;
vascular cell adhesion molecule-1
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 (VCAM-1) in human vascular smooth muscle cells (VSMCs) induced by angiotensin Ⅱ (AngⅡ). Methods The fourth to sixth passages of in vitro cultured VSMCs from human umbilical artery were used. The cells were divided into six groups, i.e. control group, AngⅡ (1?10 -6mol/L) group, Ang Ⅱ with exposure to 1, 5, 10 or 50 Gs of constant magnetic field groups. Samples were collected 12 hours after intervention. The secretion of VCAM-1 was assayed by enzyme linked immunosorbent assay (ELISA) and the expression of VCAM-1 was assessed by immunocytochemistry. Results The secretion of VCAM-1 was increased significantly 12 hours after intervention with AngⅡ of 10 -6mol/L (P
