Response of P2X receptors in CA1 region of rat hippocampus to ATP and suramin
- VernacularTitle:大鼠海马CA1区神经元P2X受体对ATP、suramin的反应
- Author:
Yandong ZHAO
;
Huaizhen RUAN
- Publication Type:Journal Article
- Keywords:
P2X receptor;
hippocampus;
patch clamp;
ATP suramin
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ATP, suramin, ivermectin (IVM) and low pH value on pyramidal cells of rat hippocampus. Methods Pyramidal cells were rapidly dissociated from the hippocampus of 7-day postnatal rats by mechanical and enzymatic methods. Effects of ATP, suramin, IVM and low pH value on P2X receptor of neurons were studied by the technique of whole cell patch clamp. Results The transmembrane current was affected by ATP and suramin. There were different effects of IVM and low pH value on ATP induced current on different neurons. Conclusion There is extensive expression of P2X receptor on hippocampus pyramidal cells of rats. The expression of P2X receptors subunits are different among neurons.
