Cardiomyocyte-induced myogenic differentiation of marrow mesenchymal stem cells
- VernacularTitle:心肌细胞介导骨髓间充质干细胞的心肌样分化
- Author:
Lei ZHANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cell;
cardiomyocyte;
differentiation
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differentiation of marrow mesenchymal stem cells (MSCs) under various in vitro conditions and analyze its mechanisms. Methods Rat MSCs were isolated and passaged. MSCs were either cultured in conditioned medium, the combination of supernatant of cardimyocyte culture and common medium, or cocultured with neonatal cardiomyocytes at the ratio of 1∶1 (cocultured group), or under normal medium (control group). After 7 d of induction, immunofluorescence was employed to detect titin, early cytoskeletal protein of cardiomyocyte, myosin heavy chain (MHC) and Connexin43 (Cx43) expression in MSCs. Results ①Conditioned medium could induce MSCs into cardiomyocyte lineage. Z part and A part of titin, large quantity of Cx43 could be detected after 1 week while myosin heavy chain was absent; ②MSCs could form junctions with neighboring cardiomyocytes. Above mentioned cardiomyocyte proteins were expressed in cocultured MSCs. Sarcomeric-like structure was detected in the cytoplasm of cocultured MSCs. Cx43 was mainly distributed in the MSC-cardiomyocyte interface. ③Untreated rat MSCs expressed myocardial gap junction protein Cx43 and titin in a lower degree. Conclusion Microenvironment of cardiomyocyte can induce the differentiation of MSCs into myocyte phenotype in vitro, either by soluble signaling molecules or by direct cell-to-cell contact. Degree of differentiation depends on the signal properties.
