Effect of low molecular N-trimethyl chloride chitosan on the proliferation of smooth muscle cells
- VernacularTitle:低分子壳聚糖季铵盐对牛血管平滑肌细胞增殖的影响
- Author:
Mei WANG
;
Hua DING
;
Xinbing WEI
- Publication Type:Journal Article
- Keywords:
vascular smooth muscle cells;
atherosclerosis;
low molecular chitosan
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of low molecular N-trimethyl chloride chitosan(LMTC) on the growth of bovine vascular smooth muscle cells(BVSMCs) in vitro. Methods The antiproliferation activities of LMTC were evaluated in BVSMCs by means of crystal violet staining and MTT assay. The morphological changes of LMTC in BVSMCs were observed under transmission electron microscope. Cell survival ratio influenced by LMTC was assessed by flow cytometer. [WTHZ]Results After BVSMCs were treated by LMTC for 72 h,the growth of the cells was inhibited in vitro and was dependent on concentration; there were some changes of apoptosis by transmission electron microscope and flow cytometer. Conclusion LMTC can inhibit the proliferation of BVSMCs and induce their apoptosis.
