Effect of apigenin on hippocampus neuron injury induced by A?-amyloid 25-35
- VernacularTitle:芹黄素对A?_(25-35)诱导损伤的海马神经元的保护作用
- Author:
Yuhong ZHAO
;
Ren HUANG
;
Jie XU
;
Yao XIE
- Publication Type:Journal Article
- Keywords:
apigenin;
amyloid;
hippocampal;
superoxide dismutase;
Bcl-xl
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of apigenin on A?_ 25-35 induced injury in primary cultured hippocampal neurons.[WTHZ]Methods[WTBZ] The primary cultured hippocampal neurons were exposed to 10 ?mol?L-1 A?_ 25-35 for 24 h after pretreatment with the apigenin of concentrations of 5,10,20,40 ?mol?L-1 for 2 h;then the survival rate of hippocampal and SOD activity were examined;we also analysed the expression of apoptosis-related gene bcl-xl with Western blot.Result The survival rate of hippocampal neurons were decreased after pretreatment with 10 and 20 ?mol?L-1 apigenin for 2 h,the increases of Mn-SOD activity and bcl-xl protein expression were also observed.Conclusion Apigenin has the bioactivity of protecting the neuron from cytoxic induced by A?_ 25-35 in cultured hipocampal neurons as estrogen.The mechanism is likely related with activating the SOD and increasing bcl-xl protein expression,which plays a pivotal role in protecting cells from apoptosis.
