Effect of transforming growth factor-?1 on activity of connective tissue growth factor gene promoter in HK-2 cells
- VernacularTitle:转化生长因子?1对肾小管上皮细胞中结缔组织生长因子基因启动子活性的影响
- Author:
Nan CHEN
;
Qing ZHAO
;
Weiming WANG
;
Jian LU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor?1;
Connective tissue growth factor;
Mitogen-activated protein kinases;
Signal transduction
- From:
Chinese Journal of Nephrology
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor-?1 (TGF-?1) on the activity of connective tissue growth factor(CTGF) gene promoter in human renal proximal tubular epithelial cell line HK-2. Methods The regulation fragment of 5' flanking region of human CTGF gene was linked to pGL3-Basic vector. The recombinant plasmid pCTGF-luc was transient transfected to HK-2 cells. The activity of CTGF promoter after treatment of TGF-?1 and mitogen-activated prontein kinases (MAPK) pathway inhibitors was assayed by means of luciferase reporter gene assay system. Results TGF-?1-induced increase of CTGF promoter activity was concentration-dependent, with a plateau at 5 ng/ml by 1.82-fold vs control (P
