Establishment of hepatic stellate cell activated model by acetaldehyde in precision-cut liver slices
- VernacularTitle:精密肝切片中乙醛活化肝星状细胞模型的建立
- Author:
Xiaoqian WU
;
Hui WANG
;
Yu GUO
;
Zhangxiu LIAO
;
Yong WU
- Publication Type:Journal Article
- Keywords:
precision-cut liver slice;
acetaldehyde;
hepatic stellate cell;
activate;
?-SMA
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To activate hepatic stellate cells (HSC) in vitro in precision-cut liver slices (PCLS) stimulated by acetaldehyde for studying and screening anti-fibrotic drugs. METHODS: PCLS were prepared by the vibratome, and incubated with 700 ?m?L~-1 acetaldehyde for 0, 2, 4 and 6 h. The medium and homogenate were retained to determine glutathione S-transferase (GST) activity, lactate dehydrogenase (LDH) leakage and hydroxyproline (Hyp) content. PCLS were prepared for paraffin sections. The expression of ?-smooth muscle actin (?-SMA) was evaluated by immunohistochemistry, and the result was analyzed with image analysis software. RESULTS: The leakages of GST and LDH were increased significantly compared with those in 0 h group (P
