Effects and mechanisms of pidotimod on expression of TNF-? and IL-6 in immune-depressed mice spleen
- VernacularTitle:匹多莫德对免疫功能缺陷小鼠脾组织TNF-?和IL-6表达及相关免疫药理作用的研究
- Author:
Hui LI
;
Bingli WANG
;
Yiqing MAO
;
Yi ZHANG
;
Zhihong LI
;
Shulan SHEN
;
Huanran TAN
- Publication Type:Journal Article
- Keywords:
pidotimod;
immunocompetence;
macrophages;
cytokines;
TNF-?;
IL-6
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the influence of injection agent of pidotimod on function of the immune-system in normal and immune-depressed mice, and to investigate the mechanisms of the immunomodulating action of pidotimod. METHODS: The expressions of TNF-? and IL-6 in mice spleen was detected by RT-PCR method. The effects of pidotimod on phagocytosis functions of mice peritoneal exudate cells was investigated by neutral red phagocytosis. The lymphocyte proliferations induced by Con A or LPS were detected by MTT method. Adopt the serum haemolysis to measure the production of serum antibody. RESULTS: The normal and immune-depressed mice were treated for 14 days with different dosages of pidotimod by injection. Pidotimod can significantly increase the expressions of TNF-? and IL-6 of spleen, potentiate phagocytosis of the peritoneal exudate cells, potentiate the lymphocyte proliferations ability induced by Con A or LPS. CONCLUSION: Pidotimod can potentiate amelioration normal and immune-depressed mice immunesystem function and increase expressions of TNF-? and IL-6 of spleen.
