EFFECT OF CONJUGATED LINOLEIC ACID ON LIPID METABOLISM AND EXPRESSION OF RELATIVE GENES IN OBESE RAT FED WITH HIGH FAT DIET
- VernacularTitle:共轭亚油酸对饮食诱导肥胖大鼠脂代谢及相关基因表达的影响
- Author:
Xiaorong ZHOU
;
Changhao SUN
;
Rong LIU
- Publication Type:Journal Article
- Keywords:
conjugated linoleic acid;
fatty acid transport protein;
acyl-CoA synthase;
PPAR?;
insulin resistance
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the effect of conjugated linoleic acid (CLA) on lipid metabolism and genes expression of fatty acid transport protein(FATP)and acyl-CoA synthase(ACS) in white adipose tissue of rats. Method: Male Wistar rats were randomly divided into control group, high-fat group and high fat +CLA group (0.75,1.50,3.00 g/100g bw). By reverse transcription polymerse chain reaction (RT-PCR) technique the expression levels of FATP, ACS and peroxisome proliferator-activated receptor-?(PPAR?) mRNA were detected. Results: Serum FFA,insulin and glucose levels of rats fed with high fat diet were higher than those of control group. Supplement of CLA can decrease serum FFA, insulin and glucose levels of obese rats, and increase expression of FATP,ACS and PPAR?mRNA in adipose tissue of obese rats. Conclusion: CLA can improve insulin resistance of obese rat and increase expression of FATP,ACS mRNA, possibly acting through activating PPAR?.
