INFLUENCE OF RETINOIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS
- VernacularTitle:视黄酸和硷性成纤维细胞生长因子对神经干细胞增殖和分化的影响
- Author:
Hong DENG
;
Fei ZOU
;
Haiji LUO
- Publication Type:Journal Article
- Keywords:
retino ic acid;
basic fibroblast growth factor;
neural stem cells
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of retinoic acid (RA) and basic fibroblast growth factor (bFGF) on neural stem cells’(NSCs) proliferation and differentiation from new born rats’ pallium. Method: NSCs were isolated from the brains of new born Sprague-Dawley rats’ pallium, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on survival and proliferation of cells were determined by MTT assay. The effects of bFGF and RA on differentiation of NSCs were observed. Results: MTT assay indicated the proliferation of cells in bFGF group, bFGF+RA group and RA group was continually increased, highest in bFGF group. The percentage of neurons differentiated from NSCs in RA group was 2 or 3 times that in bFGF group and control group . Conclusion: bFGF is important to the proliferation and long-term living of NSCs. It can prohibit the differentiation of NSCs. RA can counteract the effects of bFGF and also promote NSCs to differentiate into neurons in vitro.
