In utero ethanol exposure alters the mitochondrial protein expression of fetal mouse cerebrum
- VernacularTitle:宫内乙醇暴露对胎鼠大脑线粒体蛋白质组的影响
- Author:
Yajun XU
;
Guoqing ZHANG
;
Yong LI
- Publication Type:Journal Article
- Keywords:
Ethanol;
Proteome;
Mitochondria;
Uterus;
Mice
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To probe the effect of prenatal ethanol exposure on the mitochondrial protein expression in fetal mouse cerebrum. Methods:Pregnant CD-1 mice were given 5.0 g/kg ethanol intragastrically from pregnant days (PD) 6-15. Fetal cerebral mitochondria were isolated on PD 18. The overall mitochondrial protein was applied to two-dimensional gel electrophoresis. Differentially expressed protein spots were cut off and identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Activities of respiratory chain complex Ⅳ(72.3%?4.6% of control)and ATP synthase, and ATP content in the fetal cerebral cells were measured. Results:Expressions of some constructional and functional proteins were altered by in utero ethanol exposure. Activities of respiratory chain complex Ⅳ and ATP synthase (80.3%?5.1% of control)were both decreased. ATP content (67.9%?3.9% of control) in the cerebral cells was reduced in the in utero ethanol exposure fetuses. Conclusion:In utero ethanol exposure affects the mitochondrial protein expression of fetal mouse cerebrum, even in the tissue without obvious morphological malformations, which may be a possible mechanism of behavioral defects found in children with prenatal ethanol exposure.
