Organotypic cerebral slice culture and the course of neuronal development in it
- VernacularTitle:大鼠脑片的器官型培养及其中神经元的发育规律
- Author:
Mei ZHENG
;
Dongsheng FAN
- Publication Type:Journal Article
- Keywords:
Organ culture;
Brain;
Neurons;
Disease models,animal
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method of organotypic cerebral culture.So as to pave the way for building some neurodegenerative disease models.Methods:Organotypic cerebral cultures were prepared from prefrontal brain of neonatal SD rats. After culturing 7 to 14 days, 3 weeks, 4 weeks and 8 weeks, respectively, cerebral slices were fixed, dehydrated and sectioned in cryostat. The sections proceeded with Nissl staining and neurofilament high molecular weight (NFH) immunohistochemical staining.The difference was observed between controls and cultured slices using normal rats as controls. Results:Nissl staining showed that pyramidal neurons in cultured slices were increased in volume and lightened in staining. The delaminating construction was clear from 1 to 4 weeks after culturing. In cultured slices, immunohistochemical staining showed that NFH positive pyramidal cells appeared on layer Ⅴ on the tenth day and on both layers Ⅴ and Ⅲ after culturing 12 days. In the control group, NFH positive pyramidal cells appeared on layer Ⅴin 5-day-old rats, and appeared on both layers Ⅴand Ⅲ in over 3-week-old rats. In cultured cerebral slices, the number of pyramidal neurons on layer Ⅴ in M1 area was invariable from 12 days to 2 months. Conclusion:Orgaotypic cerebral culture can be used to study postnatal development for neocortex and build some in vitro models for neurodegenerative diseases.
