In situ hybridization analysis of mRNA expression of 5-Hydroxytryptamine_4 receptor in intestinal mucosa mast cells
- VernacularTitle:大鼠小肠黏膜肥大细胞5-羟色胺_4受体mRNA的表达
- Author:
Yan CHI
;
Xinguang LIU
- Publication Type:Journal Article
- Keywords:
Receptors,serotonin,5-HT4;
RNA,messenger;
Mast cells;
In situ hybridization
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To verify 5-HT4 receptor mRNA expression in intestinal mucosa mast cells (IMMC). Methods: IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT4 receptor mRNA in IMMC. Results: Evidently positive staining was shown in the cytoplasm of IMMC. Conclusion: We have verified the expression of 5-HT4 receptor mRNA in IMMC for the first time and provided evidence for further research.
