Effect of TNF-? on murine osteoclast differentiation
- VernacularTitle:肿瘤坏死因子?对小鼠破骨细胞分化的影响
- Author:
Yuming ZHAO
;
Lihong GE
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-?;
Osteoclast;
Bone marrow cells;
Survival
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The purpose of this study was to investigate the effect of TNF-? on osteoclast differentiation in primary murine bone marrow cell culture with and without RANKL. Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 ?g/L) with different concentrations of TNF-? (0, 1, 10, 100 ?g/L) for 5 days, the formation of TRAP(+) multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 ?g/L) and M-CSF (25 ?g/L) with or without 10 ?g/L TNF-? for 4, 5, 6 and 9 days. The number of TRAP(+) multinucleated cells and resorption pits on dentine slices were counted under light microscope. Results: In the absence of RANKL, TNF-? was unable to induce osteoclast formation from murine bone marrow precursors. In the presence of RANKL, TNF-? augmented osteoclastogenesis and bone resorption, and this effect occurred only on the early stage. Conclusion: TNF-? enhances RANKL-induced osteoclast formation and function, but can’t substitute for RANKL. TNF-? stimulates osteoclast differentiation, but not survival.
