Growth property of rat hair follicle stem cells cultured in serum-free K-SFM in vitro
- VernacularTitle:无血清K-SFM培养条件下大鼠毛囊Bu lge细胞生物学特性的研究
- Author:
Gang FU
- Publication Type:Journal Article
- Keywords:
hair follicle;
bulge cell;
cell culture;
differentiation
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study the change of growth property of rat hair follicle stem cells cultured in serum-free K-SFM in vitro. Methods Rat hair follicle bulge cells were isolated and cultured in two different conditions, serum-free K-SFM and DMEM/F12 with 10% fetal calf serum. Growth property, colony-forming efficiency (CFE), and K19 expression were analyzed. Results The CFE and positive expression of K19 were significantly higher in bulge cells cultured in serum-free K-SFM than that of DMEM/F12 with 10% fetal calf serum (P
