Effect of SVCⅢ on the proliferation and apoptosis of human cutaneous T-cell lymphoma Hut-78 cells
- VernacularTitle:蝎素SVCⅢ对人皮肤T细胞淋巴瘤细胞系Hut-78增殖及凋亡的影响
- Author:
Zhongwei TIAN
- Publication Type:Journal Article
- Keywords:
SVCⅢ;
cutaneous lymphoma cells;
apoptosis;
MTT
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibition and apoptosis of the human cutaneous T-cell lymphoma cell lines Hut-78 by traditional Chinese medicine SVCⅢ. Methods After Hut-78 cells were treated with SVCⅢ of different concentration, the inhibition and apoptosis of Hut-78 cells was determined by MTT, agarose gel electrophoresis of DNA fragment and FACS. Results SVCⅢ could inhibit remarkably Hut-78 cells growth and DNA ladder was seen by agarose gel electrophoresis. The proliferation of Hut-78 cells were inhibited in G_1 stage by FACS. Conclusion SVCⅢ can promote growth retardation and apoptosis of human cutaneous T-cell lymphoma cell lines Hut-78, which suggests SVCⅢ has antineoplastic function.
