Effects of delayed transplantation of bone marrow cells on the expressions of bcl-2/bax mRNA and proteins in myocardial cell in rat
- VernacularTitle:心肌梗死后延迟骨髓细胞移植对大鼠心肌细胞bcl-2/baxmRNA及蛋白表达的影响
- Author:
Dongxing MA
;
Huiliang LIU
;
Yongzhen ZHANG
- Publication Type:Journal Article
- Keywords:
cell transplantation;
myocardial infarction;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of delayed transplantation of bone marrow mononuclear cells (BM-MNCs) after myocardial infarction (MI) on the expressions of bcl-2/bax mRNA and proteins in myocardial cells, and to explore the possible mechanism of cardiomyocyte apoptosis. Methods The MI rat model was reproduced by ligating the left anterior descending coronary artery. Two weeks later 5?106 of BM-MNCs were injected into the infarct zone and the peri-infarct zone (BMT group). TUNEL was used to determine the cardiomyocyte apoptosis, immunohistochemical method was employed to detect the expressions of bcl-2 and bax protein, and the technique of hybridization in situ was applied to assess the changes in of bcl-2/bax mRNA expression. Results TUNEL results indicated that apoptosis index of BMT group was lowered significantly compared with the control group (4 weeks: 0.095?0.017 vs 0.173?0.018; 8 weeks: 0.0916?0.014 vs 0.182?0.015, P
