Assessment of Her-2/neu status in breast cancer: a comparison of fluorescence in situ hybridization and immunohistochemistry
- VernacularTitle:荧光原位杂交与免疫组化检测乳腺癌组织Her-2/neu基因扩增或表达情况的比较
- Author:
Mengran CAO
;
Rongcheng LUO
;
Qiang ZUO
- Publication Type:Journal Article
- Keywords:
genes, erbB-2;
breast neoplasms;
immunohistochemistry;
in situ hybridization, fluorescence
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare two assay methods, namely immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), in the detection of Her-2/neu status in mammary cancer tissues. Methods In 20 samples IHC technique was applied to detect the Her-2 protein in the cytomembrane of mammary cancer, and FISH was used to assess the amplification of Her-2/neu gene, and SPSS 10.0 software was employed to analyze the relationship between the two methods. Results The coincident rate of two methods was 85%, therefore there was a significant positive correlation between the two techniques (?~2=80.00, P=0.00). Conclusion The two assay methods, IHC and FISH showed a good coincidence in the detection of Her-2/neu status in mammary cancer tissues.
