Mechanism involved in the apoptosis of human hepatocelluar cancer cell line HepG2 induced by proteasome inhibitor MG132
- VernacularTitle:蛋白酶体抑制剂MG132诱导HepG2细胞凋亡及其机制研究
- Author:
Hui HE
;
Fang GUO
;
Shunlin QU
;
Zhong REN
;
Junwen LIU
;
Xiangdong YANG
- Publication Type:Journal Article
- Keywords:
ubiquitin-proteasome pathway;
proteasome inhibitor;
HepG2 cell;
apoptosis;
Caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of proteasome inhibitor MG132 on expression of Caspase-3 and UPP associated genes in the apoptosis of human hepatocelluar cancer cells.Methods HepG2 cells were treated with MG132 (2,5,10 ?mol?L -1) for 24 h. The apoptotic cells were determined with flow cytometric analysis. The levels of E1, E2, E3 and Caspase-3 mRNA expression were detected with RT-PCR. Caspase-3 protein expression was detected with immunohistochemistry. Results The results showed that the increase of the degree of HepG2 cell apoptosis was concentrationly dependent. RT-PCR showed that E1, E2 and E3 gene expressions were decreased in the treatment group. MG132 down-regulated the gene expression of E1, E2, E3 and up-regulated the gene/protein expression of Caspase-3. Conclusion Proteasome inhibitor MG132 may induce HepG2 cells apoptosis by inhibitting UPP activity,up-regulating the gene/protein expression of Caspase-3.
