Influence and significance of intestinal trefoil factor on Bim and Bcl-xl gene expression in neonatal rats with necrotizing enterocolitis
10.3760/cma.j.issn.1673-4408.2010.04.002
- VernacularTitle:肠三叶因子对新生鼠坏死性小肠结肠炎Bim和Bcl-xl基因表达的影响及意义
- Author:
Yarui ZHOU
;
Binghong ZHANG
;
Caixia YAN
;
Haixia ZHANG
;
Rihong ZHAO
- Publication Type:Journal Article
- Keywords:
Intestinal trefoil factor;
Necrotizing enterocolitis;
Bim;
Bc1-xl
- From:
International Journal of Pediatrics
2010;37(4):339-341,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the influence of intestinal trefoil factor(ITF) on Bim and Bcl-xl gene expression in neonatal rats with necrotizing enterocolitis(NEC),and to discuss the protective machanism of ITF on NEC.Methods Thirty neonatal rats were divided randomly into control group,NEC group and ITF group.NEC group were given intraperitoneal injection of saline 0.2 ml after NEC model of neonatal rats were established.ITF group were given intraperitoneal injection ITF 0.2mg after NEC model of neonatal rats were established.On the 4th day,all the subjects were put to death.We made HE stainting of the slice and made a histopathological examination and immunohistochemical method to detect Bim and Bc1-xl genes expression,and make image analysis.Results The pathological lesions indicated that intestinal tissue necrosis was severe in NEC group,which median was 3 point,but obviously lessen in ITF group,which median was 1 point,with ITF interfering.Image analysis showed the NEC group Bim gene expression (7.87 ± 0.14) higher than those in the control group (2.15±0.28) and ITF group (3.27±0.34),there were significant differences between 3 groups(P<0.05).Bcl-xl gene expression(11.23±0.22)in ITF group was higher than that in control group(1.89±0.28) and NEC group(2.51±0.13),there were significant differences between 3 groups(P<0.05).Conclusions Intestinal injury was ameliorated after ITF was injected intraperitoneally,ITF may protect the intestinal injury of neonatal rats with NEC by changing the Bim gene and Bc1-xl gene expresstion ratio.
