Effect of TRF1,TRF2 and telomeric stability on As_2O_3 induced MGC803 cell apoptosis
- VernacularTitle:As_2O_3诱导MGC803细胞凋亡中TRF1、TRF2蛋白表达及端粒稳定性的作用
- Author:
Yang ZHANG
;
Xiaoqiu LIANG
;
Qi SU
;
Ying SONG
;
Jianguo CAO
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
gastric cancer;
MGC803 cell;
telomeric repeat binding factor;
apoptosis;
telomere
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of As_2O_3(arsenic trioxide) on the expressions of telomeric repeat binding factor1,2 and telomeric stability of human gastric cancer MGC803 cells, and explore the mechanism of cell apoptosis. Method MGC803 cell growth inhibition was measured with MTT assay. Apoptosis was analyzed with flow cytometry. Influence on chromosome distal end was analyzed with chromosome end-end fusion analysis. Expressions of TRF1 and TRF2 were determined with Western blot analysis. Results MTT assay showed that As_2O_3 clearly inhibited the growth of MGC803 cells, depending on time and dosage. The apoptosis rates were significantly higher than those of the control group in a concentration and time-dependent manner. These changes were not found in the control group. After disposal with 5 ?mol?L -1 As_2O_3, chromosome fusion rate was obviously increased in 48 h. After 48 h of disposal with 5 ?mol?L -1 As_2O_3, the TRF1 of MGC803 cells was up-regulated while TRF2 was down-regulated. Conclusion As_2O_3 induced chromosome fusion and MGC803 cells apoptosis through up-regulating expressin of TRF1 and down-regulating expressin of TRF2.
