Interaction between nimodipine and growth factors during formation of new retinal vessels
- VernacularTitle:尼莫地平与生长因子对视网膜新生血管形成的相互作用
- Author:
Yi KONG
;
Lirong HAN
;
Yajun PENG
;
Li TANG
;
Changxiu CHEN
- Publication Type:Journal Article
- Keywords:
retinal neovascularization;
vascular endothelial growth factor;
platelet-derived growth factor;
nimodipine
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the interaction between the calcium channel antagonist nimodipine and growth factors (vascular endothelial growth factor [VEGF] and platelet-derived growth factor[PDGF]) during the formation of new retinal vessels. Methods: The hyperoxia model was induced by proliferative retinopathy (OIR) in newborn Sprague-Dawley (SD) rats. SD rats (2 d after birth) were randomized into 5 groups: normal control group, pure OIR group and group 3, 4 and 5, where the animals received retrobulbar injection of nimodipine 10 ?l, 5 ?l and 2 ?l once every 2 d for 3 times, respectively. Both eyeballs of newborn rats were made into common pathological sections and detected by immunohistochemistry method to count the nuclei of proliferative retinal vessel cells and to investigate the expression of VEGF and PDGF in retina. Results: The nuclei of proliferative retinal vessel cells and the expressions of VEGF and PDGF in pure OIR group increased significantly compared to those of normal control group(P
