Effect of CAP-administration on respiratory function and cytochrome c oxidase avtivity in mitochondria from brain cortex of rats exposed to hypoxia
- VernacularTitle:缺氧复合氯霉素处理对大鼠脑皮质线粒体氧化呼吸功能及COX活性的影响
- Author:
Rong SONG
;
Junze LIU
;
Lifeng CHEN
- Publication Type:Journal Article
- Keywords:
hypoxia;
choramphenicol;
mitochondria;
cytochrome c oxidase
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the changing aspects of CAP-administration on mitochondrial oxidative phosphorylation function and cytochrome c oxidase (COX) activity during hypoxia exposure and their mechanisms. Methods Except the control group (C group), adult male Wistar rats were exposed to a hypobaric chamber simulated 5 000-meter high altitude for 23 h every day for 0, 1, 5, 15, 30 d (H_ 0, 1, 5, 15, 30 ) respectively and administrated CAP (100 mg/kg, intraperitoneal injection) every 12 h for 7 d before sacrificed by decapitation. Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode and polarography, respectively. Results As compared with C group, mitochondrial state 3 respiration (ST_3) and respiratory control rate (RCR) and oxidative phosphorylation rate (ORP) and COX activity in H_0+CAP group all decreased significantly, but by prolonging hypoxia exposure increased and restored to the control level. Conclusion Mitochondrial respiratory function, oxidative phosphorylation efficiency and COX activity in rat brain could improve by administrating CAP during hypoxia exposure and almost reach to the control level by prolonging hypoxia exposure.
