Mitochondrial DNA deletion on the growth and invasiveness of human lung cancer cells
- VernacularTitle:线粒体DNA缺失对人肺癌细胞生长和侵袭能力的影响
- Author:
Xianlong LING
;
Yinglin LU
;
Zhiyan DU
- Publication Type:Journal Article
- Keywords:
mitochondria;
mitochondrial DNA;
lung cancer;
malignant phenotype
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between mitochondrial DNA deletion and malignant phenotypes of human lung cancer cells. Methods Two rho? derivatives of 95C and 95D were generated by treating the cultured cells with ethidium bromide. Agarose colony formation assays and Transwell invasion assays were carried out to detect the phenotypes of colony formation and invasiveness of the cultured cells, respectively. Cell growth was determined by MTT. Results The partially mtDNA-deleted cells exhibited stronger capacity of colony formation and invasiveness, and faster growth rates than their respective parental cell lines. Conclusion Mitochondrial DNA deletion might play a role in the formation of malignant phenotypes of human lung cancer.
