Inhibition effects of basic fibroblast growth factor on c-fos gene expression after tractive spinal cord injury in rats
- VernacularTitle:碱性成纤维细胞生长因子对大鼠牵张性脊髓损伤后c-fos基因表达的影响
- Author:
Lei LIU
;
Kanglai TANG
;
Jianzhong XU
;
Qihong LI
- Publication Type:Journal Article
- Keywords:
traction;
spinal cord injury;
c-fos gene;
basic fibroblast factor
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of basic fibroblast growth factor on c-fos gene expression in spinal cord neurons of rats after tractive spinal cord injury. Methods The animal model of SD rats with tractive spinal cord injury was established through tracting T_ 13-L_2 spine longitudinally with a special spinal tractor that was put on parapophysis of T_ 12-L_3 vertebrae after exposing T_ 13-L_2 spinal cord via dual laminectomy, meanwhile spinal cord function was monitored by cortical somatosensory evoked potential (CSEP). Forty model rats were randomly divided into 2 groups, the bFGF treatment group (n=20) with bFGF injection into the subarachnoid cavity and the control group (n=20) with saline injection. Five SD rats without any treatment served as normal control. The c-fos gene expression was detected with immunohistochemistry and analyzed quantitatively with image system of computer. Results The c-fos gene expression in tractive spinal cord injury was higher than that in normal control group, especially increased markedly in damaged neurons. The peak value of c-fos gene appeared in 2 h after the injury, but c-fos level in the bFGF treatment group was lower evidently than that in the control group at the different sacrificed time (P
