A novel and bionic scaffold of nucleus pulposus tissue engineering
- VernacularTitle:仿生髓核组织工程细胞支架的构建及初步理化指标分析
- Author:
Changqing LI
;
Yue ZHOU
;
Chuanzhi ZHANG
- Publication Type:Journal Article
- Keywords:
collagen type Ⅱ;
hyaluronic acid;
6-chondroitin sulfate;
scaffold;
tissue engineering;
nucleus pulposus
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design and construct a new bionic scaffold of nucleus pulposus tissue engineering. Methods The collagen-hyaluronic acid (HyA) matrices were prepared, and mixed with Collagen type Ⅱ (pH 1-2) and lyophilized. 6-chondroitin sulfate (CS) was covalently attached to collagen using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Afterwards the initial structure of bionic scaffolds was evaluated. Results The bionic scaffolds were porous structure, of which the pore size was 89-132 ?m, the porosity was 94.8%. Water-binding capacity of resulting matrices was 79.2%, and the denaturation temperature was 74.6 ℃. Glycosaminoglycan (GAG) content was 224 mg/g, and the elastic modulus was 0.673 MPa. Conclusion Composition and tissue structure between the scaffolds are similar to the extracellular matrices of nucleus pulposus. The bionic scaffold is an ideal scaffold material of nucleus pulposus tissue engineering in structure. Further experiment is required on the biocompatibility of the novel scaffolds.
