Localization of dopamine- and cAMP-regulated phosphoprotein in mouse kidney
- VernacularTitle:DARPP-32在小鼠肾脏中的表达分布
- Author:
Hua ZHAO
;
Ping GE
;
Jianping WANG
;
Haiying WEN
;
Hengjin LI
- Publication Type:Journal Article
- Keywords:
DARPP-32;
kidney;
sodium ion
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and localization of dopamine- and cAMP-regulated phosphoprotein of 32 000 (DARPP-32) in mouse kidney tissue. Methods The cellular localization of DARPP-32 in mouse kidney tissue was detected by immunoblot and immunohistochemistry. Results DARPP-32-like immunoreactivity was detected in the medullary thick ascending limb of the loop of Henle, the cortical proximal convoluted tubule, and collecting ducts in medullary rays. The renal tubules were enriched of Na+, K+-ATPase for sodium reabsorption. Conclusion The participation of DARPP-32 is a likely crucial step of the signal-transduction pathway of dopamine regulation on sodium reabsorption in renal tubule cells.
