Detection of WFS1 gene mutations in the Chinese pedigree with low frequency sensorineural hearing loss
- VernacularTitle:WFS1基因在低频听力减退家系中的突变筛查
- Author:
Qiong LIU
;
Rongguang WANG
;
Qingzhong LI
- Publication Type:Journal Article
- Keywords:
hearing loss, sensorineural;
low frequency sensorineural hearing loss;
genes, WFS1;
mutation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the mutations of candidate WFS1 gene in the Chinese pedigree with Low frequency sensorineural hearing loss. Methods PCR were performed with fourteen pairs of primers in the coding sequence of WFS1 gene. The PCR products were subsequently sequenced in the 38 individuals of LFSNHL family for screening the gene mutations. Results The PCR amplification fragments showed well quality in the five pairs of primer, and further analysis with frequencing showed no polymorphism and mutations in the members. Conclusion The present study rule out the possibility that the deafness gene WFS1, which locates on the 4p16, lead up to the hearing loss of LFSNHL pedigree.
