Antineoplastic effect of vincristine-loaded intact human erythrocytes in vitro
- VernacularTitle:长春新碱载体红细胞的体外抗肿瘤作用
- Author:
Jiang WU
;
Baohua QIAN
;
Shuxun LIU
- Publication Type:Journal Article
- Keywords:
erythrocytes;
drug carriers;
vincristine;
K562 cells;
cell cycle;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Vincristine-loaded intact human erythrocytes on tumor cell in vitro. Methods VCR was loaded in intact human erythrocytes using a modified hypotonic preswelling technique. The proliferation of K562 cells after incubation with VCR-loaded erythrocytes was detected by MTT. Cellular cycle and apoptosis of K562 cells were analyzed through FACS staining with PI/Rhodamin123, and morphology of apoptotic K562 cells and their nucleus were observed through staining with Hoechest33258. Results VCR-loaded erythrocytes could efficiently inhibit the proliferation of co-cultured K562 cells and induce the cells′ apoptosis in a dosage-dependent manner. Cellular cycle analysis demonstrated that proportion of K562 cells in G 0/G 1 decreased whereas in S+G 2/M increased after incubation with VCR-loaded erythrocytes. Such effects could also be displayed from alone VCR group, and significant change was observed in K562 cells co-cultured with unloaded erythrocytes (P
