Effects of erythropoietin on proliferation and differentiation of neural stem cells originated from adult rats brain
- VernacularTitle:红细胞生成素(EPO)对脑源性神经干细胞增殖与分化的调控作用
- Author:
Hongbo GUO
;
Fei ZOU
- Publication Type:Journal Article
- Keywords:
erythropoietin;
neural stem cells;
cell culture;
cell differentiation;
hippocampus
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To approach the influence of erythropoietin on proliferation and differentiation of neural stem cells originated from the hippocampi of adult rats in vitro. Methods Neural stem cells were isolated from the hippocampi of adult rats and cultured in vitro with serum-free incubation and single-cell cloning technique; immunochemistry technique was employed to identify the neural stem cells and neural stem cells-derived progeny. Erythropoietin (EPO) were added to cultures in different concentrations during proliferation and differentiation of neural stem cells, respectively. Results Compared with the control group, EPO supplementation group displayed an average twofold to fourfold increase in Nestin positive cells over a wide range of plating densities during proliferation, whereas showed a striking decrease in Nestin positive cells during differentiation,following an early appearance of Tuj1 or GFAP positive cells. The percentage of Tuj1 positive cells increased significantly to 57% in EPO supplementation group versus 47% in control group (n=12, P
