Analysis of the relationship between dilated cardiomyopathy and angiotensin converting enzyme gene polymorphism and P myosin heavy chain gene.
- VernacularTitle:扩张型心肌病与血管紧张素转换酶基因多态性及?肌球蛋白重链基因关系的研究
- Author:
Deling ZOU
;
Fengxian YUAN
;
Jinjin GUO
- Publication Type:Journal Article
- Keywords:
Cardiomyopathy, dilated Angiotensin converting enzyme ?myosin heavy chain Gene
- From:
Chinese Journal of Practical Internal Medicine
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the relationship between angiotensin converting enzyme gene polymorphism and ? myosin heavy chain gene and dilated cardiomyopathy (DCM) among people of Han nationality in Liaoning province. Methods The insertion/deletion (I/D) polymorphism of ACE gene was detected by the polymerase chain reaction (PCR) in 43 patients with DCM and 53 normal controls. The exons 13,14 of cardiac ?-MHC gene from 43 patients and 53 controls were amplified restrictively by using PCR. The amplified products of PCR were digested by restrictive en-donuclease DdeⅠ and NlaⅢ to identify the mutations at No. 8848 and 9124 nucleotide of ?-MHC gene. Results The frequency of ACE DD genotype and the frequency of the allele D was remarkably higher in individuals with DCM than those in normal control people ( P
