Effects of Yiqi Huoxue Ruanjian Jiedu drug serum on Ca~(2+) and mitochondrial membrane potential in BEL-7402 cell line
- VernacularTitle:益气活血软坚解毒方含药血清诱导BEL-7402细胞内Ca~(2+)、线粒体膜电位的变化
- Author:
Dongtao LI
;
Yingxia PEI
;
Xin QI
;
Guizh SHUN
- Publication Type:Journal Article
- Keywords:
drugs,Chinese herbal;
liver neoplasms;
Ca 2+;
mitochondrial membrane potential
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Yiqi Huoxue Ruanjian Jiedu(YHRJ) drug serum on Ca 2+ and mitochondrial membrane potential (△?m) in human hepatocellular carcinoma cell line BEL-7402.Methods: YHRJ decoction was composed of Astragalus Root, White Atractylodes Rhizome, Radix Notoginseng, Fructus Polygoni Orientalis, Radix Actindiae Argutae, Flos Campsis, Pangolin Scales, Oldenlandia diffusa Roxb, and Herba Scutellariae Barbatae. Twelve New Zealand rabbits were randomly assigned into 3 groups: NS group, YHRJ equivalent dose group(YHRJD) and YHRJ high dose group(YHRJG), which were fed with NS,YHRJ equivalent dose decoction (5 times as large as normal human dose) and high dose YHRJ decoction(10 times as large as normal human dosage), respectively. Drug serum was prepared after the rabbits were fed with drugs for 5 times. BEL-7402 cells were divided into 6 groups according to different drugs, including NS group, NS+DDP group, YHRJ equivalent dose group, YHRJ equivalent dose+DDP group, YHRJ high dose group, and YHRJ high dose +DDP group. The concentrations of NS,YHRJD and YHRJG were adjusted to 100 ml/L ,and the concentration of DDP was adjusted to 4.5 mg/L. Flow cytometry was applied to detect the concentrations of Ca 2+ and △?m 8, 12, 24 and 48 h after the drugs were added.Results: The Ca 2+ concentrations at 12, 24 and 48 h after treatment were significantly higher than those of the control group(P
