Regulation of nitric oxide on glucocorticoid receptor function in rat alveolar macrophages treated by LPS in vitro
- VernacularTitle:一氧化氮调节脂多糖致炎大鼠肺泡巨噬细胞糖皮质激素受体功能的研究
- Author:
Pin QIAN
;
Fang ZHANG
;
Guisheng QIAN
;
Weizhong CHEN
- Publication Type:Journal Article
- Keywords:
nitric oxide donor;
glucocorticoid receptor;
NF-?B;
transcriptional activation activity
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of nitric oxide donor (SNP) on glucocorticoid receptor function in condition of cell inflammation. Methods After fluorescence expression plasmid pGFP-GR transfected rat alveolar macrophges (AMs), nuclear translocation of GFP-GR following to lipopolysaccharide (LPS) and SNP treatment was observed through fluorescence microscope. The transcriptional activation activity of GR was evaluated by the method of relative activity of luciferase. Electrophoretic mobility shift assays (EMSA) were used to measure the activity of NF-?B. Results GFP-GR showed nuclear translocation in 2 h after nitric oxide donor (500 ?mol/L SNP) treatment, and the transcriptional activation activity of GR increased and the activity of NF-?B decreased remarkedly. The phenomena of depressed activity of NF-?B disappeared after the addition of GR special antagonism RU486. Conclusion Nitric oxide donor (500 ?mol/L SNP) exerts anti-inflammatory effect through GR activation in condition of cell inflammation.
