Effects of cantharidin on the expressions of NF-?B(P65), FAK protein and the level of phosphorylated FAK of highly metastatic ovarian carcinoma HO-8910PM cells
- VernacularTitle:斑蝥素对高转移卵巢癌细胞HO-8910PM中NF-?B(P65)、FAK表达及FAK磷酸化水平的影响
- Author:
Taiping HE
;
Lier MO
;
Nianci LIANG
- Publication Type:Journal Article
- Keywords:
cantharidin;
protein expression;
ovarian carcinoma
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of cantharidin on the expression of NF-?B(P65), FAK protein and the level of phosphorylated FAK, and to investigate posssible mechanism of cantharidin on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells. Methods MTT assay was used to examine the effect of cantharidin on proliferation of HO-8910PM cells after 24 h treatment; The expression levels of NF-?B(P65), FAK protein and the level of phosphorylated FAK were assessed by Western blot analysis.Results The expression level of of NF-?B(P65) protein decreased obviously in HO-8910PM cells treated with 5～20 ?mol?L -1 cantharidin for 24 h, and the effects appeared in a dose-dependent manner. Cantharidin significantly up-regulated the expression of FAK, down-regulated the level of phosphorylated FAK.Conclusion The antitumor activity of cantharidin is involved in the expression of NF-?B(P65) and the level of phosphorylated FAK.
