Trinitrobenzene sulfonic acid induces experimental colitis in Balb/c mice
- VernacularTitle:三硝基苯磺酸诱导Balb/c小鼠结肠炎的实验研究
- Author:
Xueqin YUAN
;
Xudan WANG
;
Ming XIE
;
Zeji QIU
;
Yong ZHOU
;
Yun XU
- Publication Type:Journal Article
- Keywords:
trinitrobenzene sulfonic acid;
mouse;
colitis
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish experimental colitis model in Balb/c mice by Trinitrobenzene Sulfonic Acid (TNBS) enema. Methods Several doses of TNBS instilled into mice colon induced experimental colitis, then mortality rates of mice were observed. Severity of colitis was evaluated by the Disease Activity Index (DAI),Morphologic and Histologic analysis and Myeloperoxidase (MPO) analysis. We also observed the T cell proliferation of spleen. Results It showed that the mice mortality rate was increased when the mice were given the higher dose of TNBS. Most survived mice showed chronic inflammation in reduction colon. Histological examinations of the colon showed multiple erosive lesions and inflammatory cell infiltration composed of macrophages, lymphocytes, neutrophils in lamina propria and beyond mucosal layer. Some colon showed crypt distortion or reduction and high vascular density. Conclusion A TNBS dose of 1.5mg for each mouse was chosen for an appropriate experimental dose since the group showed less mortality rate and appropriate experimental colitis.
