Construction,expression and purification of HIV-1 p24-gp41 fusion protein in E.coli
- VernacularTitle:HIV-1 p24和gp41融合基因表达载体的构建及融合蛋白的表达纯化
- Author:
Weimin JIANG
;
Hongzhou LU
;
Xiaozhang PAN
- Publication Type:Journal Article
- Keywords:
HIV core protein p24;
HIV envelope protein gp41;
Gene expression;
Recombinant fusion proteins
- From:
Chinese Journal of Infectious Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinant plasmid with p24 and gp41 gene, express fusion protein in E.coli. Methods To design primer with restriction endonuclease position,and amplify p24 and gp41 by RT-PCR, link both into pMD18-T vector.To choose correct clone with target gene.Then p24 fragment will be cleaved and linked into pMD18-T vector within gp41 gene. Both post-linked gene will be cleaved and linked into pET21a vector. The vector will be transformed into E.coli. And protein is highly effective expressed in E coli. Western blotting proved that the expressed product could react with 6?his antibody. Result Fusion protein p24-gp41 is highly effective expressed in E.coli. Conclusion Fusion protein p24-gp41 is highly effective expressed in E.coli in pET21a vector.
