Effects of angiotensin type-1 receptor blockade on expression of angiotensin-converting enzyme 2 in renal of spontaneously hypertensive rats
- VernacularTitle:血管紧张素受体阻滞剂对自发性高血压大鼠肾脏血管紧张素转换酶2表达的影响
- Author:
Liu YANG
;
Changhui LIU
;
Yi WANG
;
Yupeng BAI
- Publication Type:Journal Article
- Keywords:
spontaneously hypertensive rat;
angiotensin-converting enzyme 2;
renal;
angiotensinII;
irbesartan
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of angiotensin-converting enzyme 2 (ACE_2) in the renal of spontaneously hypertensive rats and the effects of angiotensin type-1 receptor blockade. METHODS: Twenty of 14-week-old male SHR were randomly divided into two groups (n=10 in each): SHR group and irbesartan group. Ten of 14-week-old male WKY group were served as control. Irbesartan group were given a daily dose of 50 mg?kg -1 irbesartan for 12 weeks by gavage and the other two groups were fed with a normal diet. Angiotensin-converting enzyme 2 was examined by immunohistochemistry and RT-PCR. RESULTS: ACE_2 in the renal was significantly down-regulated in the SHR group compared with the WKY group ( 0.72? 0.11 vs 1.11? 0.15). After 12 weeks treatment with irbesartan, the attenuated ACE_2 expression in irbesartan group was markedly enhanced compared with the SHR group ( 1.03? 0.13 vs 0.72? 0.11). CONCLUSION: Hypertension induces the reduction of ACE_2 in the renal in rats and irbesartan markedly increases ACE_2 expression. It suggests that up-regulation ACE_2 which is one of counter-regulatory particular components in the renin-angiotensin system may be a new mechanism of curing hypertension beyond blockade angiotensin type-1 receptor.
