Expression of antibacterial peptide gloverin in vitro
- VernacularTitle:利用RTS500系统进行抗菌肽葛佬素蛋白的体外表达
- Author:
Ping LUO
;
Bo XU
;
Yongling LU
;
Hong ZHOU
- Publication Type:Journal Article
- Keywords:
gloverin;
antibacterial peptide;
rapid translation system;
expression;
PCR
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To construct a recombinant vector containing gloverin and express it in vitro by Rapid Translation System(RTS500). METHODS: The gloverin cDNA was amplified by PCR and inserted into the prokaryotic expression vector pIVEX2.3. The recombinant product was identified by PCR and enzyme digestion. The positive reconstructed expression plasmid pIVEX2.3-G was expressed by RTS500 in vitro. The expressed protein was identified by SDS-PAGE and western blotting. RESULTS: Positive recombinant plasmid pIVEX2.3-G was successfully constructed. Target protein of 13.8 Kda with 6-his tag was detected by SDS-PAGE and western blotting. CONCLUSION: Gloverin polypeptide is successfully expressed in inactive E.coli in vitro.
