EFFECT OF ?-3 PUFA ON THE PROLIFERATION AND APOPTOSIS OF TUMOR CELLS TRANSFECTED WITH TRANSMEMBRANE TNF? EXPRESSION VECTOR
- VernacularTitle:?-3PUFA及其诱导的外源性跨膜型TNF?对肿瘤细胞生长的影响
- Author:
Hai CHENG
;
Mantian MI
;
Qianyong ZHANG
- Publication Type:Journal Article
- Keywords:
?-3 PUFA;
transmembrane TNF?;
apoptosis;
tumor
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of ?-3 PUFA on the proliferation and apoptosis of tumor cells transfected with transmembrane TNF? (tmTNF?) expression vector containing peroxisome proliferator responsive element (PPRE). Methods:The tmTNF? expression vector was transfected into HL-60 and MCF-7 tumor cells. The effects of ?-3PUFA and/or exogenous tmTNF? on cell proliferation, cell cycle distribution and apoptosis were measured by growth curve,flow cytometry and DNA ladder assay. Results: The production of exogenous tmTNF?gene was located in the cell membrane. After treatment with?-3 PUFA, the expression of exogenous tmTNF? in transfectant was significantly increased. The proliferation was decreased in HL-60 and MCF-7 cells treated with 6.0?10-5 mol/L ?-3 PUFA,but cell cycle was arrested at G0/G1 and the DNA ladder was detectable only in HL-60 cells. These changes were more obvious in transfected cells than non-transfected cells. Conclusion:?-3 PUFA and the product of exogenous tmTNF? gene induced by ?-3 PUFA could inhibit tumor cell growth more effectually,and induction of apoptosis may play a key role in this process.
