EFFECT OF ZINC DEFICIENCY ON THE GENE EXPRESSION OF VITAMIN D RECEPTOR AND CaBP IN DUODENAL MUCOSA OF RATS
- VernacularTitle:缺锌对大鼠小肠粘膜维生素D受体及钙结合蛋白基因表达的影响
- Author:
Xiaodan YU
;
Chonghuai YAN
;
Xiaoming SHEN
- Publication Type:Journal Article
- Keywords:
zinc deficiency;
vitamin D receptor;
CaBP;
gene expression
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of zinc deficiency(ZD)on the gene expression of vitamin D receptor(VDR)and CaBP in duodenal mucosa of rats. Methods: Thirty weaning male rats were randomly divided into three groups:ZD,paired-fed(PF),zinc adaquate(ZA). After 15 d feeding, took the duodenal mucosa to extract RNA,and measured the levels of VDR mRNA and CaBP mRNA in duodenal mucosa by real time fluorescent quantity PCR. Results: The VDR gene expression of ZD group was downregulated 88.89% as that of PF group,and 50.00% as that of ZA group. CaBP gene expression of ZD group was downregulated 80.16% as that of the PF group,and 88.50% as that of ZA group. Conclusion: Zinc deficiency, by changing the activity of VDR and the mRNA expression of VDR,affects the transcription of the target gene CaBP,and then the absorption of calcium that causes allo-osteogenesis.
