EFFECTS OF POLYSACCHARIDE FROM LYCIUM BARBARUM ON CELL APOPTOSIS AND bc1-2 GENE EXPRESSION IN IRRADIATED MICE
- VernacularTitle:枸杞多糖对慢性辐射小鼠细胞凋亡及bc1-2基因表达的影响
- Author:
Deyuan LI
;
Jian TANG
;
Xianbo XU
;
Xisheng SU
;
Fei ZHONG
;
Jinyi ZHONG
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharide(LBP);
cell apoptosis;
bcl-2;
gene expression
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of polysaccharide from Lycium barbarum (LBP) on cell apoptosis and bc1-2 gene expression in irradiated mice. Methods:Forty eight Kunming mice were divided into three groups (LBP group, radiation group and normal group). LBP chow was prepared through adding 0.8%LBP to normal chow and was supplied to LBP group. Normal chow was supplied to normal group and radiation group. LBP group and radiation group were exposed to whole-body 60Co ?-rays at the dose of 0.084 Gy/day for 6 w, five times a week and the total dose was 2.52Gy. Then the micronucleus frequency of polychromatic erythrocytes(MF),chromosome and sperm aberration frequency, caspase-3 mRNA expression, cell apoptosis and bcl-2 gene expression were detected. Results:LBP could significantly lower MF, chromosome and sperm aberration frequency, and cell apoptosis , and it could increase the proliferation activity of bone marrow cells and bc1-2 gene expression in irradiated mice and decrease caspase-3 mRNA expression.Conclusion:The radioprotective effect of LBP is related to regulation of cell apoptosis and bc1-2 gene expression.
