An in vitro study of cytotoxic and antineoplastic effect of Solanum nigrum L extract on U266
- VernacularTitle:龙葵总提取物对多发性骨髓瘤U266细胞株的作用
- Author:
Wei WANG
;
Daopei LU
- Publication Type:Journal Article
- Keywords:
Solanum nigrum L;
Multiple myeloma;
Cell cycle;
Apoptosis
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study cytotoxicity and antineoplastic effect in vitro Solanum nigrum L extract on U266. Methods: U266 cells were cultured together with the extract of Solanum nigrum L. Cytotoxicity assay was tested by CCK-8. Cell cycle and apoptosis were determined using flow cytometry (FCM) analysis. Results: Extract of Solanum nigrum L showed strong cytotoxicity against U266 cells. The IC_ 50 was about 117 mg/L. After exposure of U266 cells to the drug for 48 hours, the cell cycle distribution was changed compared with the controls. There was decrease of cells in the G_0/G_1 phase with increase of cells in the S phase and G_2/M phase. Apoptosis of U266 cells could be shown with staining of Annexin V FITC/PI or TFAR19 testing through FCM. The proportion of apoptotic cells increased in parallel with the increase of the drug dosage. Conclusion: Solanum nigrum L extract showed strong cytotoxicity effect on U266 cells. The antineoplastic effect of the drug can partly be ascribed to its apoptotic inducing effect.
