Effects of estrogen, progestin and mifepristone on endometrial carcinoma cell lines
- VernacularTitle:雌、孕激素和米非司酮对子宫内膜癌细胞的作用
- Author:
Ye LU
;
Li YU
;
Chunling CHEN
;
Qinping LIAO
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Estrogen;
Progestins;
Mifepristone;
Receptor,progesterone
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of estrogen, progestogen and mifepristone on endometrial carcinoma cell lines in vitro. Methods: The well-differentiated endometrial cancer cell line Ishikawa and moderate-differentiated endometrial cancer cell line Hec-1B were cultured in vitro. The cells were divided into four groups:control, estrogen , estrogen and progestogen , estrogen and progestogen and mifepristone, then we implanted cells in 96-well plates to search the response in distinct hormones. Results: When stimulated by estrogen for 72 hours, the growth of Ishikawa cells was significantly higher than the control, Hec-1B cells only grew higher than the control, but it was no significance in statistics. Progestogen inhibited Ishikawa cells significantly after being stimulating for 72 hours, there was the same effect on Hec-1B cells after being stimulating for 96 hours. On the base of estrogen and progestogen, we added mifepristone,cells developed after 96 hours in Ishikawa cells and cells developed after 48 hours in Hec-1B cells. Conclusion: Estrogen can cause endometrial carcinoma cell growth; progestogen inhibits the hyperplasia induced by estrogen; mifepristone antagonizes the effect of progestogen on cell growth.
