Interleukin-6 is involved in the enhanced proliferation of HEK293 cells by the tumor specific antigen of HCA520
- VernacularTitle:白细胞介素-6参与肿瘤相关抗原HCA520对HEK293细胞的促增殖作用
- Author:
Xiaoping QIAN
;
Kejun HAN
;
Weifeng CHEN
- Publication Type:Journal Article
- Keywords:
Antigens, neoplasms;
Cell line;
Cell division;
Cytokines;
Interlenkin-6
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of tumor specific antigen of HCA520 on the proliferation of HEK293 cells and to obtain some functional implications for HCA520. Methods: MTT assay was performed with HEK293 stable cell lines transfected with pcDNA3-HCA520-flag construct. Cytokines probably involved in HCA520 enhanced proliferation were screened by RT-PCR, and effect of these cytokines on the HEK293 cell proliferation was further confirmed by MTT assay. Results: HCA520 significantly promoted the proliferation of HEK293 cells, which was at least partially attributed to the up-regulation of IL-6 in HEK293 cells by HCA520. Conclusion: HCA520 might accelerate tumorigenesis by promoting proliferation of cancerous cells.
