Transfection of T-STAR antisense gene can depress the telomerase activity in lung adenocarcinoma cell line A549
- VernacularTitle:反义T-STAR基因转染降低肺腺癌A549细胞端粒酶活性
- Author:
Ling ZHANG
;
Lian GUO
;
Yong PENG
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
T-STAR;
human lung adenocarcinoma cells;
telomerase;
cell transfection
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of telomerase activity after knocking down endogenous expression of T-STAR (testes-signal transduction and activator of RNA) gene in human lung adenocarcinoma cell line A549 by antisense strategy. Methods The mRNA and protein expression of T-STAR gene were determined by RT-PCR and Western blotting, and the telomerase activity was measured by PCR-ELISA, after transfection of T-STAR antisense gene into A549 cells with lipofectamine. Sense pcDNA-STAR and blank pcDNA3.1 transfection served as control. Results The expression of T-STAR gene was significantly inhibited at mRNA and protein level, and the telomerase activity was significantly decreased. Conclusion The down-regulation of telomerase activity may result from inhibition of T-STAR gene expression in lung adenocarcinoma A549 cells.
