Establishment of mice model that induces mucosal immunity by oral infection of Toxoplasma gondii
- VernacularTitle:经口感染弓形虫诱导小鼠黏膜免疫动物模型的建立
- Author:
Hongli LIU
;
Jianfeng HAN
;
Guorong YIN
;
Guohua SONG
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Mice;
Animal model;
Mucosal immunity
- From:
Chinese Journal of Schistosomiasis Control
1989;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish mice model that induces mucosal immunity by orally infected tachyzoites of Toxoplasma gondii. Methods Respectively, 5?103, 5?104, 5?105, 5?106 tachyzoites of RH strain were inoculated to BALB/c mice by stomach delivery. The control group was given PBS solution. Symptoms and pathological changes of mice were observed. Secretory im-munoglobulin A (SIgA) was assayed. The lymphocytes from Peyer's patches (PP) and intraepithe-lial lymphocyte (IEL) were observed and the changes of CD4+ and CD8+ T cells assayed by SABC immunohistochemistry. Results Inoculation of 5?104 tachyzoites of Toxoplasma gondii caused symptoms and pathological changes in mice. The titre of SIgA increased in intestine, and CD4+ T subset of the mucosal inductive sites and CD8+ T subset of the mucosal effectors' sites increased. Conclusion Mucosal immunity may be induced by oral infection of 5?104 tachyzoites of T. gondii RH strain in BALB/c mice.
