Comparative proteomics studies in gastric cancer from serum by SELDI TOF mass spectrometry
- VernacularTitle:胃癌患者血清比较蛋白质组学研究
- Author:
Chunfang GAO
;
Donghui LI
;
Guan ZHAO
- Publication Type:Journal Article
- Keywords:
stomach neoplasm;
tumor marker, biological;
surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI TOF MS)
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for the biomarker used to determine gastric cancer by the application of protein mass spectrometry analysis. Methods The relative contents of serum proteins were detected of 38 patients with gastric cancer and 82 healthy people by IMAC3 (CipherGen Inc.) chip and proteinchip. Results At the M/Z values range from 1 723Da to 14 048Da, 18 kind of protein contents are obviously different between the two groups. In the learning mode, all the 120 testers were correctly distinguished, both the sensitivity and specificity reached to 100%. While in the test mode, 31 patients and 81 control people were correctly distinguished, the accuracy were 81 6%(31/38) and 98 8%(81/82), respectively. Conclusion Gastric cancer can be quickly and exactly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application
